Protein folding refers to the process and path by which a nascent polypeptide obtains its 3-D or native state. Over the years the field of protein folding has evolved, as are the questions pertaining with this perplexing field. Levinthal, was first to propose that the folding code for a polypeptide to fold, is its primary structure or sequence of amino acids. But, still in present era, a major challenge is to predict the native structure of a protein solely from its sequence. This has become more challenging with ever increasing sequence data being accumulated with each day. Thus, protein folding problem continues to be a complex problem to be solved and has perplexed scientists over the decades. In this article, main focus will be to sum up the broad aspects of protein folding, protein denaturation and discuss in brief the methods to estimate protein stability.

Keywords: Protein Folding; Denaturation; Stability; Equilibrium Methods; Differential Scanning Calorimetry.