Background: It is very important to identify the local bacterial population and their antibiotic sensitivity pattern. This study aims at finding out local bacteria causing positive blood cultures in neonatal sepsis. Objective: To identify the pattern of growth of micro organisms in the blood culture in patients of neonatal sepsis in a tertiary care centre of India. Material and Method: A prospective randomised study was carried out in the Emergency Department of Peerless Hospital and B K Roy Research Centre Kolkata, India for one year (01/07/2013 to 31/06/2014) results: A total of 30 samples were found positive where This study shows that klebsiella.p and candida is the most common organism causing sepsis in maximum type of samples of neonates in this tertiary care centre of Eastern India. For Klebsiella.sp the first choice of antibiotics should be amikacin, colistin, tigecycline. And second choice should be Cefepime and Carbapenams. For Candida sp the first choice of antibiotics should be Voriconazole and Flucytocin. Second choice should be Fluconazole and Amphotericin B. Staph was most sensitive to Linezolid(100%), Tigecycline (100%), and Vancomycin(100%) Enterobacter(20%) is most sensitive to Colistin and Tigecyclin(100%) ,than Amikacin, Cefepime, Cefoperazone and Sulbactum,Piptaz and Meropenam all 50% sensitive. Conclusions: This study paves a path for future studies to occur.

Keywords: Neonate; Blood Culture; Sepsis.