This study was conducted to observe the effect of Pseudomonas aeruginosa extract on different human blood cells with different interval of time. Venous blood was collected from laboratory personnel in EDTA tube. Pseudomonas aeruginosa was isolated from different clinical samples. 20 different isolates were cultured on fresh nutrient agar after 24 hours of incubation at 370C. Isolates were centrifuged. The bacteria were resuspended in cold Sterile Distilled Water (SDW) and disrupted by sonication on ice for three times after 1 min intervals. Extract filtered through 0.22 µm Millipore Millex. Extracts were incubated with human blood. After  incubation time, blood was used to observe effect on extract, on cells in cell counter. This study shows the effect of Pseudomonas aeruginosa extract on human blood cells.   In this study, 20 Non-duplicate isolates of Non-MDR, MDR and PDR Pseudomonas aeruginosa were tested. Blood Sample (Heparinized venous blood) was collected in EDTA tube from healthy personnel. Bacterial isolation and further processing of extraction of toxin was done as per the standard procedure. Significant difference of Pseudomonas aeruginosa extract of all three groups was seen on human blood cells. Effect of extract was same on blood cells at particular interval of time.