AbstractCancer is a major public health burden in both developed and developing countries. Anticancer activity is the effect of natural/synthetic/biological/ chemical agents to reverse, suppress or prevent carcinogenic progression. Several synthetic and natural origin compounds are used to cure the disease but they also show toxicity and hence the research is going on to find new compounds and/or to improve upon the existing anticancer agents. Therefore an attempt has been made to review different in vitro and in vivo methods for estimating anticancer properties of synthetic and natural compounds.
Keywords: Tumor; Carcinoma; Assay; MTT Assay; InVitro; InVivo; Dye.