Aims: The present study is to evaluate the in vitro Anti-diabetic activity of the various extracts of Adhatoda vasica Nees on glucose uptake by isolated rat hemi-diaphragm. Methods: The Adhatoda vasica Nees was extracted by using n-hexane, chloroform, ethyl acetate, methanol, and aqueous. The different extracts were then used to study on in vitro glucose uptake by isolated rat hemi-diaphragm. Results: The ethyl acetate and methanol extracts of the plant extracts showed significant glucose uptake when compared with control. The result strongly suggest that Adhatoda vasica nees will be alternative choice for treatment of diabetes mellitus. Conclusion: In the in vitro uptake assay in rat hemi diaphragm, Ethyl acetate and Methanol extract and the isolated compound showed significant of glucose uptake (41.3653±0.657188, and 27.26±0.88) respectively. The results suggested that the extracts and compound enhanced the uptake of glucose by isolated rat hemi diaphragm which was more effective than insulin.

Keywords: Adhatoda Vasica Nees; Glucose Uptake by Isolated Rat Hemi-Diaphragm; Diabetes Mellitus, Nilgiris.