Background and Aims: Propofol is widely used for induction of anaesthesia, although the pain during its injection remains a concern for all anaesthesiologists. A number of techniques have been adopted to minimise propofol induced pain. Various 5 hydroxytryptamine 3 antagonists have shown to reduce propofol induced pain. Hence, this placebo controlled study was conducted to compare the efficacy of ramosetron and lignocaine in terms of attenuation of propofol induced pain during induction of anaesthesia. Methods: Hundred adult patients, aged 18–60 years, posted for various elective surgical procedures under general anaesthesia were randomly assigned to two groups of 50 each. Group R received 0.3 mg of ramosetron, Group L received 0.5 mg/kg of 2% lignocaine. After intravenous (IV) pre treatment of study drug, manual occlusion of venous drainage was done at mid arm with the help of an assistant for 1 min. This was followed by administration of propofol LCT after release of venous occlusion. Pain was assessed with a four point scale. Unpaired Student’s t test and Chi square test/ Fisher’s exact test were used to analyse results. Results: In our study out of 50 patients of each study group, 54% in lignocaine group and 60% in ramosetron group did not have pain, 34% in lignocaine group and 24% in ramosetron group had mild pain, 10% in both groups had moderate pain, 2% in lignocaine group and 6% in ramosetron group had severe pain. Conclusion: Pre treatment with IV ramosetron 0.3 mg is equally effective as 0.5mg/kg of 2% lignocaine in preventing propofol induced pain.