Background: In comparison to open surgery laparoscopic cholecystectomy is beneficial. Nevertheless, pneumoperitoneum and the alteration because of patient position causing pathophysiological offers limitation in anaesthetic management. We designed a study to observe the changes on heart rate and blood pressure, intraocular pressure and end­tidal carbon dioxide pressure due to use of clonidine and nitroglycerin during laparoscopic cholecystectomy. Method: The study included 60 patients belonging to American Society of Anesthesiologists class I­II, planned for elective laparoscopic cholecystectomy were randomly divided into 2 groups: Clonidine infusion was given in group 1 at the rate of 1.5 µg/kg/hr and Group II received nitroglycerine infusion at the rate of 0.5 µg/kg/min (30µg/kg/hr). The parameters observed included mean arterial blood pressure, heart rate, end tidal CO2 , and intraocular pressure. Results: The groups were comparable with no significant differences in gender and age. Heart rate of the patients were observed at preinduction, at intubation and after intubation at at interval of 5 minutes onwards upto reversal. Heart rate comparison after 15 minutes onwards upto reversal between group I and II showed statistically significant difference. Mean arterial pressure comparison between group I and II showed insignificant difference. Mean IOP at the time of induction in group I and II was 16.27±3.06 and 18.13±2.80 respectively, which was significant statistically. ETCO2 difference between the groups was not statistically significant. Conclusion: The findings in our study suggested that clonidine surpass nitroglycerin in effectiveness with regard to prevent hemodynamic parameters changes and IOP induced by insufflations of CO2 in laparoscopic cholecystectomy. Also there is no significant hypotension requiring stopping of infusion or further treatment.