Question: Is there any significant difference in strengthening of gluteus maximus and rectus femoris on dynamic and static balance? Design: Experimental design comparative in nature. Participants: Thirty asymptomatic participants (mean age 21.39±1.99) of Sardar Bhagwan Singh Institute, Dehradun, India, with normal body mass index and right dominant lower extremity, were divided randomly in three groups of 10 participants in each. Intervention: Two week (5 sessions a week) close kinematic chain strengthening program of gluteus maximus (group c) and rectus femoris (group b) where gluteus medius (group a) is controlled. Outcome measures: Single limb stance time, star excursion balance test (sebt) in eight (Anterior, Anteriomedial, Medial, Posteriomedial, Posterior, Posteriolateral, Lateral, Anteriolateral) directions. whereas in sebt improvement for group c was greater (8.26±4.30, 7.13±4.48, 7.25±4.0, 10.62±5.31, 6.9±6.05, 8.38±3.11, 6.38±3.54) compare to group b (4.63±4.47, 6.63±3.81, 5.63±3.81, 4.5±1.85, 10.5±5.78, 7.5±5.4, 5.13±4.36, 4.5±3.02) and group a (2.25±3.57, 2±3.02, 2±3.30, 1.75±5.36, 2±5.04, 3.25±5.25, 2.62±5.85, 3.25±2.66) in all eight (Anterior, Anteriomedial, Medial, Posteriomedial, Posterior, Posteriolateral, Lateral, Anteriolateral) directions. Conclusion: This study provides idea that the strengthening program of rectus femoris and gluteus maximus has the effect on static as well as dynamic balance as the improvement of single limb balance and SEBT was documented in both the groups. Data analysis revealed that improvement was more in group in comparison to group B and group A (C>B>A) which stated that gluteus maximus strengthening has better effect on static (single limb stance) and dynamic (SEBT) balance in comparison to rectus femoris strengthening.