Food is an important and basic biological need of man. Food is the foundation of good health. it is a good culture medium and a potential carrier of infection, Cleanliness and care should be maintained in handling. There is need for mothers to understand the knowledge, attitudes, practices of on food safety. The objective of the study was to assess the level of knowledge regarding food hygiene among mothers with under five children in Maraimalai Nagar. Quantitative approach and descriptive survey design was adopted for the study. A total of 40 samples were selected using non probability purposive sampling technique in Maraimalai Nagar. The instrument used for the study comprises of 2 sections, section A was demographic data which includes age, religion, educational status, income per month, type of drinking water and section B was a structured questionnaire developed by the investigator which included 30 questions to assess the knowledge regarding food hygiene among mothers with under five children. The data was collected from the 40 samples and the analysis was done using descriptive and inferential statistic. The study findings concluded that among 40 mothers 18(45%) mothers had moderate knowledge and 17(42.5%) mothers had adequate knowledge on Food hygiene which implies that nurses should play a vital role in enhancing the knowledge on food hygiene among under five mothers.