he quality of skin wound healing can be improved by the application of collagen scaffolds as biological dermal substitutes. Dermal extract helps to improves wound healing and quality of the scars. They serve as a scaffold into which cells can migrate and repair the injury. In the current scenario where in many biological and cellular engineering skin substitutes are available, wound management is a multimodality treatment with use of multiple available methods to augment wound healing at various levels. An excellent dermal substitute should be affordable, long-lasting, ready-to-use, analgesic, durable, flexible, non-antigenic, stops water loss, conforms to uneven wounds, anti-microbial, and may be applied in one sitting. In this study, we attempted to mimic the same technique in our two-layered regenerative scaffold, which is created locally and is cost-effective.