Quest for Antimicrobial agents from plant sources is a prime area of research in the last decade. Manjistha (Rubia Cardifolia Linn) is Popular medicinal plant in Ayurveda which has multiple indications includes Krimighna,Vishaghnaetc Krimighna property is often taken has Antimicrobial property hence present study was conducted to screen the Antimicrobial activity of Manjista Root on selected Organisms viz Staphylococcus Aureus E-Coli, Candid albicans, and Aspergillosisflavus. The study showed significant results in higher zone of inhibition in Alcoholic extract and lower in aqueous extract, and antifungal activity of aqueous extract at 500mcg Showed moderate activity for candid albicans & Aspergillosisflavus.

Key words: Rubiacordifolia; Antimicrobialactivity.