New upsurge of interest in Ayurveda and its rapidly increasing public use has given rise to many newer issues and challenges. One being lack of standardization and there is a need to develop it. Central council of Research in Ayurveda and Siddha (CCRAS) has given preliminary guidelines for standardizing formulations. This present paper reports on standardization of Dooshivishari Agada (DVA), an Ayurveda Herbomineral compound. Ingredients of DVA were procured, authenticated and prepared in classical way by giving bhavana with the qwath prepared by same ingredients of Agada and consequently analysed for Organoleptic, physical characteristics, physiochemical, phytochemical screening, TLC and HPTLC was done for standardization. The phytochemical constituents found to be present in the finished product will possibly facilitate for understanding mechanisms of pharmacological action.