Introduction: Human cadaveric dissection is most routinely practiced method of learning anatomy for medical students since many years. With the use of technological advancements in teaching and learning methods, there has a high risk of reduction in human cadaveric dissection as a method of learning in this modern digital era. Aims & Objectives: The present study was done to analyse the medical student’s preferences regarding the teaching and learning methods of human anatomy and to analyze the perceptions regarding anatomy dissection. Material and Methods: The sample constituted 346 randomly selected medical students from the medical institute of Navi Mumbai, Maharashtra. The present cross sectional study was conducted using a simple random sampling. Result: Total 346 students were participated in the present study. The mean age of the participants was 19.54 (±1.24) years. Males & females constituted 45.7% & 54.3% respectively of total study population. Dissection was ranked as the most preferred method by 246 (71.1%) by the students, followed by lectures 44 (12.7%) and textbooks 30 (8.7%) over the least preferred methods such as demonstrations 20 (5.8%) and computer assisted learning 6 (1.7%). Strong positive agreement was displayed by the students for items describing that dissection still the best method of learning anatomy 334 (96.5%) and dissection helps in feeling more comfortable with patients subsequently 332 (96%). Strong negative agreement was displayed by the students for items describing that dissection is boring and less informative method 282 (81.5%) and there are other better methods as dissection is outdated 268 (77.5%). Conclusion: This study has revealed that medical students still rated anatomy dissection as a most preferred method of learning anatomy, despite technological advancements.