Objective: The aim of this work was to investigate the acute and sub-acute toxicity studies of the homeopathic drugs, causticum, calcerea, medorrhinum, mercurius, formica, proteus, silica, sulphur, thuja in experimental models. Materials and Methods: Animals were divided into groups (n=5). Homeopathic drugs were administered to rats for 14 and 28 days to assess the toxicological profile. Acute and sub-acute studies were carried out according to OECD 425, 407 guidelines. Behavioural parameters and mortality rate were assessed in acute toxicity study. The sub-acute was performed for a study period of 28 days and afterwards animals were sacrificed to carry out biochemical and haematological estimations. The histopathological analysis of all vital organs was done with haematoxylin and eosin staining to assess the anatomical damage involved. Results: Findings of the study revealed that the administration of homeopathic drugs at single bolus dose in acute toxicity study there were no mortality or any signs of toxicity observed after oral administration of drugs up to the dose level that of 2000 μl in rats. So, the LD50 was found to be greater than 2000 ul/100g body weight. Sub-acute toxicity study was conducted for a period of 28 days and we noted that the there were no pathological or biochemical alterations found. Conclusion: Study inferred that homeopathic drugs are safe on acute and sub-acute administration for 14 and 28 days respectively. The safety profile is established in experimental animals and further data can be corroborated in humans.