Infertility is defined as inability to conceive after one year of unprotected intercourse. Male infertility is nearly half in out of 15% infertile couple. Oxidative stress is an important factor of this morbidity. DNA integrity in sperm is essential for accurate transmission of genetic information. Sperm DNA fragmentation Index (DFI) is an important parameter to get a conclusion of male infertility. On searching literature for review in Google Scholar, Pubmed and Cochrane library and after doing meta analysis, found there are four major types of tests for DNA fragmentation namely Sperm chromatin structure assay (SCSA) Comet, Tunel, Sperm Chromatin dispersion (SCD) assay. These tests have their own intrinsic merits and demerits. Therefore DFI is a valuable test to identify idiopathic infertility. It gives worth information to clinician as well as to the couple. 

Keywords: Infertility; Sperm; DNA Fragmentation; Assisted Reproductive Technologies.