Impairment of spermatogenesis and defective sperm function is the most prevalent cause of male infertility. Recent reports indicate that increasing male infertility could be due to genomic abnormalities. Therefore the present study was aimed to evaluate sperm DNA fragmentation in oligospermic infertile as well as fertile men and to study correlation of sperm DNA fragmentation with sperm parameters. The study is prospective, comprises 30 oligospermic infertile men and 30 fertile men. Sperm DNA integrity in terms of DNA fragmentation Index (DFI) was assessed by flow cytometer. Sperm DNA fragmentation was found to be significantly increased in oligospermic infertile men as compared to fertile men.  It was negatively correlated with sperm count and sperm motility. Therefore assessment of sperm DNA damage can be used to identify cause of infertility in patients with poor semen quality. This needs to be considered while treating patients with sperm quality anomalies for better outcomes of infertile patients.