A growing need for sustainable solutions is one of the primary drivers of the demand for industrial enzymes. One of the most significant and beneficial sources of many enzymes has been and still is the microbial world. Numerous industrial procedures, such as chemical synthesis used to create chemicals and pharmaceuticals, have a number of drawbacks: Lack of enantiomeric specificity for chiral synthesis, low pH, high pressure, high temperature, and low catalytic efficiency. Enzyme research and interest are still advancing, which helps industrial biocatalysis succeed even more. There should be a lot of intriguing discoveries in the field of biotransformation over the coming years. The value of biotechnologically and industrially significant microbial enzymes is the main topic of this study, which comprises 44 papers, including research studies and review articles. Also, it offers novel insights into the micro-organisms that manufacture these enzymes as well as the procedures employed for their purification and separation.