Background: The neonates are at risk for various health problems, increasing morbidity and mortality rates. Nursing personnel have important responsibilities to assess neonatal for early detection of problems and initiate prompt management. Objective: The objective was to assessthe knowledge regarding neonatal resuscitation among staff nurses working in NICUs in private and government hospitals in Punjab and to provide an informational booklet to staff nurses regarding neonatal resuscitation to reduce the infant morbidity and mortality rate. Materials and Methodology: A non-experimental comparative study design and quantitative research approach were utilized. The setting for the private hospital was Grover hospital, Amritsar and the government hospital was a civil hospital, Amritsar with a sample size of 100 staff nurses 50 from a private hospital and 50 from a government hospital selected by using a convenience sampling technique. The tool was divided into two sections- demographic characteristics and 30 self-structured knowledge questionnaires on neonatal resuscitation. The data collection was done in the month of February 2019. The Guttman method assessed the knowledge questionnaire's reliability (r=.735). Results: The results of the study revealed that of staff nurses working in private hospitals, 68% had average knowledge and of the staff nurses working in governmental hospitals 64% had average knowledge. It suggests that staff nurses working in NICU should be updated with efficient knowledge and skill in performing neonatal resuscitation...