Estimation of Time Since Death (TSD) is very important in routine forensic practice. Various techniques and parameters are utilised for determination of this TSD. One of the most recently and vastly studied method of determination of TSD is with the help of vitreous electrolyte concentration analysis. Recently workers have reported a significant between eye differences for same individual at identical TSD. This has doubted the use of vitreous electrolyte analysis in TSD determination. The review of literature highlights various causes behind this fact and need of more research in this topic as discussed in this article.

Keywords: Vitreous Electrolytes; Time Since Death; Between Eye Differences; Sampling Errors; Analytical Errors.