Biological materials’ collection, preservation and analysis are essential procedures in any Medico legal work. Improper collection of these specimens can greatly alter or negate chemical and toxicological analysis. The investigation of a death from suspected poisoning may depend upon the correctness or otherwise of sampling of fluids and tissues from the body. This article is an update about the standard methods of biological materials collection procedures, preservation and analysis which will be helpful for the Medical and forensic doctors. 

Keywords: Precaution; Collection; Preservation; Biological materials; Poison; Medico legal cases.