Tere is a growing trend in the different antibiotics against microbes. However, studies were found that a microorganism interacts at molecular level in a unique way against a range of antibiotics. Te present study was undertaken to analyze the effect of expired and unexpired drugs which are commonly used by Indians without doctor’s
prescription. Due to a change in chemical composition or a drop in potency, expired medical items may be less effective or dangerous. Expired drugs may harbor bacteria, and sub-potent medicines may even fail to treat infections, resulting in more serious diseases and antibiotic resistance. Tere is no assurance that the drug will be safe and
effective afer the expiry date. Sensitivity test and Spectrophotometry were performed for the selected expired and unexpired antibiotics and drugs - Ampicillin, Norflox-TZ, and Althorn. Te growth pattern of microbes in antibiotics before and afer their expiry was performed and then compared. It was evident that fresh forms of antibiotics
have signifcantly inhibited the growth of microbes as compared to expired drugs. Te expired forms of antibiotics lost their efcacy in expired drugs.