Cardiovascular autonomic function tests were conducted in twenty five male tobacco chewers and twenty five male non-tobacco chewers in the age group of 20-25 years. Resting heart rate showed a statistically significant increase (p value <0.05) in tobacco chewers. A significant increase (p value <0.01) in the incidence of resting tachycardia was also observed in tobacco chewers after Gutka chewing. Heart rate response to standing (Parasympathetic system test), (p value <0.01) and blood pressure response to sustained hand-grip (Sympathetic test), (p value <0.01) showed a statistically significant higher incidence of abnormality among tobacco chewers as compared to controls. 

Keywords: Cardiovascular autonomic function tests; Tobacco chewing.