Objective: The study investigated the optimum electrode placement site in mature premolar teeth and the response threshold of these teeth using electrode pulp tester and also highlighted the effect of gender and age, and arch variables on threshold values., Study Design: Eighty sound premolar teeth in 20 adults aged 18-50 years were tested using an EPT to determine the thresholds at which a response was evoked at 5 sites of each premolar tooth., Result: In both arches, the mandibular first premolar teeth responded at al over threshold while the 18- 20 years age band had the highest value. However, gender effect was not statistically significant., Conclusion; Within the limitation of the study, the report will enhance clinical practice.

Key words: Electric pulp test, premolar teeth, mean threshold response.