Background: One of the most variable muscles in the human body is palmaris longus. The donor tendon is selected for grafting is dependent on the size, length, and width of the tendon. In view of the above significance, this study is taken up with the purpose to determine the morphological variations of palmaris longus muscle. Aim: The palmaris longus muscle was studied pertaining to its: width & length of the fleshy belly, width & length of the tendon. Materials & methods: This study was conducted in Anatomy Department of following colleges: Dr B.R. Ambedkar Medical college, Bangalore, Hassan Institute of Medical Science, Hassan and Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar. A total of 60 upper limbs out of which, 25 were from males cadavers & 5 were female cadavers were dissected and study was conducted from 2011 to 2014. All the measurements of palmaris longus were taken using digital vernier calipers. esults: The length of Palmaris longus tendon 238.4 mm and width of palmaris longus tendon is 5.94 mm. The results of this study confirms that in a South Karnataka region suggest the palmaris longus tendons would be of ideal width and strength for use as grafts in reconstructive surgery. Conclusion: In our study, the width of palmaris longus tendon is 5.94 mm. Hence, tendons from upper limb should be considered when strength of width is important. The palmaris longus and plantaris tendons are the most suitable tendons in reconstructive surgeries. The other tendons which are suitable are extensors of the digits and tendons of flexor digitorum superficialis.