Soil is a very valuable evidence due to its diversity and ubiquity. It is very helpful in indicating the origin and helps in establishing relationship between suspect and crime scene. Recent studies on soil microbiology have helped to determine its origin, and diversity in soil samples have been successfully developed. Whenever the soil is transferred from one place to another and collected as a evidence, its microbial structure can also changes with time. Soil is examined on the basis of colour, texture but it can also be examined on the basis of microbiome present in it. Bacteria and fungi can be very helpful in determining the relation between the suspect and crime by various method TRFLP, CLPP, C-substrate. The technique T-RFLP is reliable and it is easily available in almost laboratories due to its use in extraction of human DNA. Also it is easy to work with and cost effective. In today era we can use soil micro biome to establish link between suspect and crime as the use of bacterial and fungal profile have shown promising result in differentiation and comparison of soil sample to a great extent. Keyword: soil, bacterial, fungal, T-RFLP.