Background: Tuberculosis is an infectious disease that primarily affects the lung parenchyma. It also may be transmitted to other parts of the body, including the meninges, kidney, bone and lymph nodes. Tuberculosis is one of the top 10 causes for death and leading cause of single infectious agent worldwide. India has managed to scale up basic TB services in the public health system. More than 10 million TB patients were getting treatment under Revised National Tuberculosis Control Programme (RNTCP). Objective: to assess the level of knowledge on Pulmonary TB treatment regimen and follow up care among Primary Caregivers’ of patients with Pulmonary Tuberculosis and to find out the association between Primary Caregivers’ knowledge on Pulmonary TB treatment regimen and follow up care with selected demographic variables. Methodology: the research approach used for this study was quantitative research approach and the design selected was descriptive survey research design, 150 subjects were selected using simple random sampling technique the data was collected using structured questionnaire. Result revealed that with respect to general information, 73 (48.67%) had moderately adequate knowledge, 72 (48%) had adequate. With regard to treatment regiment, 124 (82.67%) had inadequate knowledge, Considering the frequency, 76 (50.67%) had moderate adequate knowledge, Regarding the follow–up care, 90 (60%) had moderate adequate knowledge. The overall level of knowledge revealed that 86 (57.33%) had moderately adequate knowledge, 57 (38%) had adequate knowledge and only 7 (4.67%) had inadequate knowledge. The result revealed that the demographic variables association with educational qualification, type of family, and duration of time statistically significant at the level of p < 0.001. and family history of TB statistically significant at the level of p < 0.01. Conclusion: Caregivers’ to mandatorily have adequate knowledge regarding tuberculosis treatment regimen and follow up care to provide complete care to facilitate patient’s adherence to drug regimen and there by prevent spreading tuberculosis to achieve the goal “End TB”.