Context: The only staffs that are present always in any hospital, with or without the presence of other staff, are the nursing staff. Obviously, nursing staff are the backbones of hospitals. To perform duties properly, they should maintain physical and mental wellbeing, because any deterioration of their health would, in turn, affect the health of the patients they are attending to. So, assessment of the health of nursing staff is essential. Aims: Assessment of health of nursing staff can be done in many ways, for example, by measurement of status of the important antioxidant, vitamin E. So, aim of the work described here was to evaluate whether there was any change in serum vitamin E levels in nursing staff of different shifts, and if so, to determine the degree of significance in the change. Settings and Design: The study included sixteen nursing staff from the day shift, and age and sex-matched sixteen staff from the night shift, from the indoor patient departments. Methods and Material: Serum vitamin E levels were assayed in all subjects. Statistical analysis used: Comparison of two groups was undertaken using SPSS. Results: Serum vitamin E levels were significantly decreased in night shift nursing staff with respect to those of day shift. Conclusions: The results suggest that decrease of vitamin E levels in serum may be related to night shift duty and stress. Vitamin E may be considered as a useful biomarker of antioxidant status in night shift duty and stress, and can help in monitoring and possible remedial strategy.