Introduction: A study to assess the level of knowledge on Biomedical waste management among IV Year B.Sc .Nursing Students,  studying in College of Nursing, Madurai Medical College, Madurai. Objectives: To assess the level of knowledge on Biomedical waste management among IV Year B.Sc. Nursing Students, studying in College of Nursing, Madurai Medical College, Madurai. To associate the level of knowledge on Biomedical waste management among IV Year B.Sc. nursing students with their selected demographic variables. Conceptual framework: The conceptual framework based on Health Belief Model,  this model was created by Becker. Research approach: Quantitative approach. Design: Descriptive study design was adopted. Setting of the study: The study was conducted College of nursing, Madurai Medical College Madurai. Subjects: There were 45 subjects included in the study. Sampling technique: Purposive sampling technique was used. Findings: This study revealed that there is significant association between Residence, Education of the students, Education of the father, Education of the Mother, Occupation of the father and the level of knowledge among IV Year B.Sc. nursing students regarding biomedical waste management with their selected demographic variables. Conclusion: The study shows that the IV Year B.Sc. nursing students had adequate knowledge regarding Bio-medical waste management.