Background: Transfusion-transmissible infections such as hepatitis B virus (HBV), human immunodeficiency virus (HIV), hepatitis C virus (HCV) and syphilis are among the greatest threats to blood safety for transfusion recipients thus posing a serious public health problem. This study was done with the aim to study the Seroprevalence of transfusion transmitted infections amongst voluntary and replacement blood donors at Blood Bank and an yearly comparison was made to find out the incidence of HIV, HBV, HCV, Syphilis and Malaria. Methods: In this study, we aimed to access the prevalence and trend of HIV, HBV, HCV and Syphilis over the last 3 years (January 2015 to December 2017) among the blood donors who came to donate Blood at Blood Bank, RBM hospital and associated hospitals as well as in various blood donation camps organized by the same blood bank. Results: From the total of 34628 blood donors, 801 (2.31%) had serological evidence of infection with at least one pathogen, either of HIV, HBV, HCV or Syphilis. These included 23 (0.066%) with HIV, 705 (2.03%) with HBV, 35 (0.10%) with HCV, 47 (0.13%) with Malaria and 27 (0.078%) with Syphilis. Conclusion: From the quantum of blood donors harbouring these infections found in the study, it becomes mandatory to properly select blood donors and perform comprehensive testing of donor’s blood using standard methods to ensure the safety of blood recipients

Keywords: Seroprevalence; Voluntary; Replacement Blood Donors; HIV; HBV; HCV; Malaria; Syphilis; Transfusion Transmissible Infection (TTI).