The aim of the study is assess the knowledge regarding revised national immunization programme among mothers of under five children in selected rural areas. The objective of the study is to assess the knowledge regarding revised national immunization programme among mothers of under-five children in selected rural areas of the city and to find out the association between knowledge score with selected demo graphic variables. The conceptual framework of the study was developed on the basis of Pender’s health promotion model. Methodology non experimental descriptive approach was used. The study was carried out at selected rural areas of Nagpur. The sample comprised of 100 mothers of under five children in selected rural areas of Nagpur selected by convenient sampling. Pilot study was conducted on 10 samples and the tools were found to be reliable. Data collection was done from 4/12/2017 to 6/12/2017. Data was collected by administering a structure knowledge questionnaire. The result of this study showed that majority of frequency of mothers of under-five children found in that 6% mothers of under five children had poor level of knowledge score, 50% had average, 37% had good and only 7% had very good level of knowledge score. The association of knowledge scores with religion of mothers of under-five children. The finding of this supports the need of the health education for mothers of under five children in selected rural areas of Nagpur.