Objective: To compare the effectiveness of different Root Canal irrigants. To evaluate comparative efficacy of different root canal irrigants when used singly or in combination for the removal of smear layer and debris. Methods: One hundred fifty freshly extracted premolars, for orthodontic purposes were selected. Access cavities were prepared and working lengths were established. The teeth were divided into 6 groups of 25 teeth each. Six groups of teeth were irrigated with Saline, Sodium Hypochlorite (NaOCl) 5.2%, Chlorhexidine Gluconate (CHX) 0.2%, Hydrogen Peroxide (H2O2) 3%, Ethyldiamine Tetra Acetic Acid (EDTA) 17% and Sodium hypochlorite and EthylDiamine Tetra Acetic Acid respectively. The teeth were sectioned longitudinally and examined under Scanning Electron Microscope for removal of smear layer and debris at different levels of root canal system. Results: The best smear layer and debris removal was obtained with 5.2% Sodium Hypochlorite and 17% Ethyldiamine Tetra Acetic Acid combination followed by Ethyldiamine Tetra acetic Acid 17% when used alone. Conclusion: The intracanal irrigation was found to be most effective with a combination of irrigation rather than when used alone. 

Key words: Root Canal Irrigants; Efficacy; Smear Layer; Scanning Electron Microscope.