Triazophos is considered as Organophosphorous poison. Although several instrumental methods like UV-VIS, GC, and HPLC are available for separation and identification of Triazophos, but cost of analysis is very high. Therefore a simple, cheap, rapid and reliable Thin Layer Chromatography (TLC) method for separation of Triazophos has been presented. Triazophos was first extracted from blood and then identified on TLC plates by using various solvent systems. The detection of spots on the developed TLC plates was performed by using Bromophenol Blue followed by 4% Acetic acid as the spraying reagents. There were 9 different solvent systems with different volumetric ratios used in separation and identification of Triazophos.

Keywords: Triazophos; Organophosphorous; Solvent Systems; TLC; Rf ; Spraying Reagents.