In the present experiment effects of silver nanoparticles (AgNPs) on various heamatological parameters and total protein content of some selected tissues (i.e., muscle, liver, kidney and gill) of an Indian fresh water fish, Channa punctatus was evaluated. Two different concentrations of biologically synthesized AgNPs (100% and 50%) were intra-muscularly injected to the experimental fish is respect of distilled water treated control at different fixation intervals (viz., 6 hr, 24 hr, 72 hr, 96 hr, 7 day and 15 day). Treatment of AgNPs (100% and 50%) to the experimental fishes showed a significant decrease in number of the red blood cells and increase in number of white blood cells particularly at the longer fixation intervals (7 and 15 days) in respect of distilled water treated control. Total protein content of liver and kidney showed significant increase during 15 and 30 days of exposure compared to the control. In the contrary, total protein content of gill and muscle showed a significant decrease after exposure to AgNPs in respect of the control.