Effects of organic manure and different types of microbial biofertilizers were studied on growth and yield of Mustard (Brassica juncea). The experiment was conducted during Rabi season 2014-15 at the research field of Patanjali Bio Research Institute, Haridwar, Uttarakhand. The mustard cultivar Pusa 27 (EJ-17) was used in the study. The field experiment was laid out in Randomized Block Design (RBD) with four treatments and three replications along with one control. Different morphological parameters were taken and mean of observed value recorded. Tallest plant of size 48.4 inches was found on 65 Days After Sowing (DAS) for Treatment T3 (Carbon 24%, Nitrogen 2.25 %, Phosphorus 2.75%, Potassium 1.775 %, Calcium 3%, Magnesium 0.45%, Sulphur 0.4%, Iron 0.6%, Zinc 0.35%, Manganese 0.09%, at the rate of 101 Kg acre-1, Humic acid 4.0% at the rate of 12.7 Kg acre-1 , Amino acids 0.3% at the rate of 12.7 Kg acre-1, Lignite, Dolomite, Calcium carbonate, Trichoderma Sp., Pseudomonas Sp., Azotobactor Sp. at the rate of 760 ml acre-1) and the shortest plant oh height 39.6 inches in control. Maximum number of flower 27 in T3, whereas minimum number of flower 15.6 in control. The result showed that application of microbial biofertilizers significantly increased the total grain yield. The maximum grain yield 10.33 quintal acre- 1 was recorded in treatment T3 in comparison to 6.33 quintal acre-1 for control.