Abstract Investigation into the role of Ca2+ on amelioration of physiological disorder caused by higher Fe2+ in soil (pH5.6), was carried out with rice varieties viz., Mahsuri (iron resistant) and Bahadur (iron susceptible). Root-dip treatment (RDT) for 24h with CaCl2 increased [Ca2+] in roots commensuration with [CaCl2] from 100-1000ppm. The soil pH values increased (acidity decreased) along with advancement of crop growth. Overall, 400ppm added FeSO4.7H2O in soil reduced total chlorophyll content (up-to 46%) in leaf as compared to natural soil (200ppm Fe2+), and the CaCl2 RDT reversed (17- 45%) the negative effects of iron on total chlorophyll at 30, 60 and 90 days after transplanting (DAT). Similarly, leaf area was reduced (1-16%) by the 400ppm added iron in soil, and CaCl2 RDT recovered (12-23%) of it in comparison to the plants on natural soil. High density (HD) grains (12-23%) and yield (15-37%) were also reduced by plants grown in higher iron condition, and CaCl2 RDT could increase HD grains (32-33%) and grain yield (39- 2%) in both rice varieties. In spite of susceptibility of Bahadur to iron, it responded well to CaCl2 RDT in amelioration of iron disarray. The membrane damage by Fe2+ and modulation of it by Ca2+ have been discussed as the features of plants under higher iron condition.