Introduction: Urosepsis is a serious infection that has a high rate of morbidity and death. Improving patient outcomes requires early detection and treatment. Although automated urinalysis provides a quick and non-invasive diagnostic method, nothing is known about how well it predicts urine culture results in cases of urosepsis. With this background, we aimed to find the association between the results of urinalysis and those of concurrent urine cultures. Methodology: This observational study was conducted in the Department of Pathology at the tertiary care hospital among 200 patients who were subjected to urine cultures for suspected UTIs and they were selected through a convenient sampling method. Automated urinalysis was performed using Laura XL’s automated urine sediment analysis instrument. Data were collected and analysed using IBM SPSS v21. Fisher exact test was used to find an association between Urine analysis and urine culture. Results: Of the 200 patients with the symptoms of UTI studied, 156 patients had positive for urine culture and Automated urinalysis showed 124 of them had Leucocyte esterase and 115 of them had nitrite in urine analysis. The majority of the patients had E. coli, followed by Klebsiella species in the urine culture. Leucocyte esterase showed 89.86% sensitivity and Nitrite 89.84% sensitivity. Both Leucocyte Esterase (0.0001) and Nitrite (0.0001) were significantly associated with Bacteria present in urine culture. Conclusion: Automated urinalysis shows promise in predicting urine culture outcomes in patients with suspected urosepsis. Integrating AU into the diagnostic pathway may facilitate faster clinical decision-making, potentially improving patient care.