Ninety five numbers of female dogs with mammary tumors presented at the small animal clinics from different regions of Ludhiana during 2021 to 2023 were investigated for prevalence of the disease. Temperature and respiration rate of tumor bearing bitches were comparable to that of values of healthy dogs but the pulse rate was observed to be significantly (p<0.05) increased in the tumor group. 69.81% of cases presented were observed to be obese. Adult female dogs (5-10 years old) were most frequently affected (54.71%) followed by old aged dogs (>10 years old). Spitz breed accounted for 39.62% of cases presented followed by German shepherd (31.13%), non-descript (16.98%) and Labrador (12.26%). Female dogs from industrial areas are most susceptible to mammary tumors (79.24%). 79.24% of tumors were palpated to be hard and 20.75% are soft. Histopathlogical analysis revealed that 66.03% of tumors were malignant and 33.96% belong to benign group. Our study concluded that mammary tumor in female dogs is an emerging animal health problem and special attention is needed for its prevention and treatment.