Industrial effluents, agricultural run offs and sewage from households are regularly discharged into the derelict water bodies as well as fish culture ponds, which generates a potential risk for both human and fish. In the present study, one month survey (August, 2019) was conducted in eight different derelict ponds randomly selected from Kalyani subdivision of Nadia district, West Bengal, India to see its potential efficacy to be a fish culture pond by analyzing its physicochemical and microbiological parameters along with enzymatic assay. Different physicochemical parameters like dissolved oxygen, pH, temperature, conductivity, phosphate, nitrate, nitrite, ammonium-N, chemical oxygen demand, hardness, organic carbon and total alkalinity of the water were statistically analyzed with the abundance of heterotrophic and phosphate solubilizing bacteria in the studied ponds. High and low bacterial enzyme activity on the other hand clearly reflected the optimum and unfavouranle nutrient enrichment conditions in water. It was found that the sample data provide strong enough evidence to conclude that the bacteria count have a pressure over water quality parameter studied in different ponds particularly on phosphates and hardness of the water as the P value is less than significance level of 0.05 causing rejection of null hypothesis. The data provides additional information regarding alteration of bacterial enzyme activity in the studied ponds. Thus, the ponds warrant for adoption of proper measures to reduce the pollution level at the point source to be a fish culture pond.