The production and estimation of protease by Aspergillus flavus has been done by using cotton seeds, rice bran and sugarcane bagasse as substrates. OD was recorded at 660 nm. Protease activity and specific activity was calculated daily till 6th day of incubation. The best substrate for maximum protease production was found in sugarcane bagasse and high protease estimation has been observed between 4-7 days of incubation. The study proved that locally isolated fungal species is able to produce a very high level of protease under SSF using inexpensive agro-residual substrates, which can be useful in industries.

Keywords: Aspergillus sp.; Protease; Protein assay.