Hair is an important piece of evidence in forensic investigations. Analysis of the morphological features (medulla) of hair has been reported since the early 1800’s. However, many questions still remain unanswered especially as to, how local populations could be analysed and separated from each other based on the morphology (medulla) of their hair. In the Present Study medulla types of hair have been examined among the Ashanti and Dagomba, two important populations of Ghana by using the Comparison and Compound microscope together with Computer imaging. Statistical analysis has been performed on the data to determine the variability and relationships between the two populations. Medulla have been found to be absent in 51.5% of the Ashanti population examined while among the Dagomba it has been observed to be 33%. The difference between the two populations for their medulla types have been found to be statistically insignificant.