Advertisement!
Author Information Pack
Editorial Board
Submit article
Special Issue
Editor's selection process
Join as Reviewer/Editor
List of Reviewer
Indexing Information
Most popular articles
Purchase Single Articles
Archive
Free Online Access
Current Issue
Recommend this journal to your library
Advertiser
Accepted Articles
Search Articles
Email Alerts
FAQ
Contact Us
Journal of Forensic Chemistry and Toxicology

Volume  8, Issue 2, july-december 2022, Pages 53-61
 

Original Article

Assessment of the Potency of Ceftiofur Sodium Powder through Validated Microbiological Method

Bhavna Kumari 1 , Anil Kumar Teotia 2 , Prasad Thota 3 , Piyush Kumar 4 , Manoj Kumar Pandey 5 , Rajeev Singh Raghuvanshi 6

1 Pharmacopoeial Associate, 2 Principal Scientific Officer, 3 Scientific Officer, 4 Pharmacopoeial Scientist, 5 Senior Scientific Officer, Microbiology Division, 6 Secretary-cum-Scientific Director, Indian Pharmacopoeia Commission, Ministry of Health & Family Welfare, Ghaziabad, Uttar Pradesh 201002, Government of India

Choose an option to locate / access this Article:
90 days Access
Check if you have access through your login credentials.        PDF      |
|

Open Access: View PDF

DOI: http://dx.doi.org/10.21088/jfct.2454.9363.8222.5

Abstract

Ceftiofur Sodium is a semi-synthetic beta-lactamase broad spectrum third generation of cephalosporin antibiotic. The development and validation of a two-level agar diffusion (2+2) bioassay to quantify ceftiofur in powder for injection is described in this paper. Bacterial strain Bacillus subtilis ATCC 6633 (MTCC 441) was selected and used as the most significant strain against ceftiofur sodium. The mean potency recovery value of ceftiofur sodium in marketed sample XCEFT powder for injection was estimated to be 101.79%. All potency results were statistically analyzed and found to be linear (r2 = 0.9916) in the range of 1.0-10 µgmL-1, with the intermediate precision RSD between days was 0.81%; intermediate precision RSD between analyst was 0.43% and accuracy 101.08%, RSD = 0.28%. The findings backed up the proposed microbiological technique, which allowed for accurate ceftiofur sodium quantification in pharmaceutical samples. Furthermore, bioassay is a useful, easy and cost-effective method for controlling the quality of ceftiofur sodium in raw material as well as in pharmaceutical preparations.


Keywords : Ceftiofur sodium, Bacillus subtilis, Bioassay, Quality Control, Potency.
Corresponding Author : Anil Kumar Teotia