Background: Early childhood caries is a virulent form of dental caries that can destroy the primary dentition of infants and preschool children. ECC is a preventable disease and prevention should begin in pre and perinatal. Mothers with poor oral health may be at greater risk of infecting their children. Biologically, the mother is a primary source of Streptococci and young children are dependent on their mother for oral hygiene. Aim: The aims of this study were to assess women's knowledge and experiences of dental health in pregnancy and to examine the selfcare practices of pregnant women concerning their oral health. Methodology: A survey was presented which was designed to assess the knowledge, attitude, and practices of pregnant women regarding infants' oral health. A survey was conducted in and around Vellore at a government and private maternity hospital among pregnant women and new mothers. A self-administered questionnaire consisting of a total of 41 questions concerning basic information on proper infant oral hygiene procedures was given. The statistical analysis was performed. Results: A greater percentage of women were unaware of the importance of their oral hygiene, infant feeding practices and oral hygiene procedure. Conclusion: The present study reflects a need for maternal counselling on infant oral health. Keywords: Infant feeding, Knowledge, Mothers, Oral health.