Context: To see effect of transfusion on variants of haemoglobin in neonates. Results: There was significant difference in mean value of serum haemoglobin variants (Hb F, Hb A & Hb A2) at age of one month but no significant difference in mean value of serum haemoglobin variants at three months & six months in cases and control. Aims: To compare if there is any difference in variants of haemoglobin in transfused and non-transfused neonates at various ages. Settings and Design: Tertiary care hospital in north India. Prospective cohort study Methods and Material: All neonates who underwent double volume exchange transfusion with whole blood in neonatal period were followed up at ages one month, three months and six months for their haemoglobin electrophoresis and compared with similar parameters in age and sex matched controls. Statistical analysis used: Average and standard deviation of various variants of haemoglobin in both the groups was calculated and p value between the values of both the group was derived. Results: p value of HbF, HbA and HbA2 between cases and controls was not significant (p=0.27, p=0.21 and p=0.39 respectively) at six months of age. Conclusions: Major haemoglobin variants in transfused and nontransfused neonates equalizes by three months of age. Keywords: Blood Transfusion; Levels of Haemoglobin; Neonates in First Six Months.

Key Messages: Haemoglobin variants; In transfused and nontransfused; Neonates equalizes by three months of age