AbstractBackground: Organ and tissue transplantation has become a vital part in the health care in every country. Hence it has become the need of the hour to the knowledge and attitude of the general public towards organ donation. Purpose: The study was designed to determine the knowledge regarding organ donation among general population, to find out the attitude towards organ donation among general population, to find an association between knowledge of general population regarding organ donation and demographic factors and to find an association between attitude of general population towards organ donation and demographic factors. Methods: A cross sectional descriptive survey was designed among 100 and a questionnaire survey conducted among general population in Kerala. Results: The overall knowledge about organ donation among study participants was 97% (n=100). And 97% showed a favourable attitude towards organ donation. Conclusion/Implications for practice: majority of participants are aware about the legislation and the process of organ donation. This study shows the importance of creating awareness among the general population and conduct studies to explore the common obstacles which prevent people from volunteering for organ donation. Keywords: Organ Donation; Attitude; Knowledge.