AbstractIntroduction: Upper GI diseases (lesions) are frequently seen in clinical practice. These are associated with mortality and morbidity in patients with GI lesions. Early diagnosis of inflammatory disorders, infectious diseases, physical, mechanical and toxic reactions and neoplasms of upper gastrointestinal tract is possible with endoscopic biopsies with its histopathological correlation. Materials and methods: This prospective study was carried out on 162 patients in Dr. D.Y. Patil Medical college, Hospital and research centre, Pune for a period of 2 years from June 2019-2021. Biopsy specimens were fixed in 10% formalin and routinely processed in Haematoxylin & Eosin stain. Results: A total of 250 biopsy samples were obtained from 162 patients of which 188(75%) were from Stomach, 33 (13%) from Duodenum, 25(10%) from Esophagus and 2% from gastro esophageal junction. Maximum biopsies were carried out in the age group of 31-40 years. 149 (64.19%) of biopsies were from males and 101 (35.8%) were from females. Among the total cases, 140 (86.41%) were non neoplastic lesions whereas 22 (13.58%) were neoplastic lesions. Epigastric pain seen in 71 (43.8%) was the commonest symptom ofupper gastrointestinal lesions. Conclusion: The upper GI endoscopy helps in early detection of several mucosal lesions, diagnosis of malignancies at early stages and of clinically suspected lesions leading to prompt treatment.