Introduction: Urinary tract infection (UTI) in a very common bacterial infection in both sexes and all age groups and is also a major cause of hospitalization. E.coli is the most common causative organism in UTI with almost 85% isolates being E.coli . The main aim of antibiotic treatment is to completely eliminate the bacteria from the urinary tract. However, with the changing pattern of antibiotic sensitivity of urinary pathogens to commonly used antibiotics and the emergence of ESBL producing E.coli the situation has worsened. A new drug, Fosfomycin , has now been frequently used as an alternative drug in the treatment of uncomplicated UTI. The present study was carried out in our microbiology department with the aim of finding out the susceptibility pattern of Fosfomycin to frequently isolated urinary pathogens. Materials and Methods: This was a retrospective study and urine culture of patients from January to December 2015 were included in the study. A total of 2759 urine cultures of all ages and both sexes were included in the study. All urine specimens were inoculated on Blood and MacConkey agar plates and incubated at 370C for 24 hours .A growth of >105CFU/ml of bacteria was labelled as significant and was further processed.The bacteria were identified on the basis of Gram staining and colony morphology and accordingly the panel for identification & susceptibility was chosen to be processed on Vitek II ( (Biomerieux). Results: Out of 2759 urine cultures ,1341 (48.6%) cultures showed growth of bacteria. 1418 (51.3%) cultures were sterile .E. Coli (86.7%) was the commonest organism in followed by Klebsiella (8.50%), Pseudomonas (2.53%), Staphylococcus (1.41%),Enterococci (0.67%) and Proteus (0.14%). Highest sensitivity(100%) was observed in Proteus,followed by Pseudomonas(82.3%), Klebsiella(73.6%), Enterococci(66.6%) E.Coli(57.6%),and least for Staph.aureus(51.8%). Conclusion: The presence study suggests that resistance to Fosfomycin is on the rise and should be used with caution and resistance pattern should be monitored periodically. 

Keywords: Fosfomycin; UTI; Antibiotic Susceptibility.