Background and Objective: Health status of rural females is the most neglected in India. Hepatitis B is one of the highly infectious and sexually transmitted diseases which have a direct impact on the health of rural females as well on their siblings and family. So this study was undertaken to know the burden of Hepatitis B viral (HBV) infection in rural females so a prevention strategy can be made to curtail such dreaded infection in rural society. Method: A total number of 5035 female subjects of different age ranging from neonate to 90 years were grouped in 8 different groups designated as A to H. They were screened for detecting HBsAg both by Rapid Diagnostic tests and ELISA technique Results: Maximum number of subjects belonged to age group 21-30 yrs of group C, followed by group D(31-40yrs) and B(11-20 yrs). Of 5035 subjects, 256(5.08%) were positive for HBsAg. The positivity was maximum in both the extremes of life, 7.8%% & 21.9 t & 19.5% percent respectively. In other group the HBs Antigen positivity varied from 2.5 to 5.8 percent. Interpretation and Conclusion: The high incidence of Hepatitis B Viral infection clearly reflects the uneducated and neglected health status of rural females. Prevention strategy and education can reduce the further transmission and thereby reducing morbidity and mortality with HBV infection. 

Keyword: HBsAg; HBV; HBV in Females.