Silver nanoparticle synthesis needs reduction. Seeds of plant are known to exude reducing agent during imbibition. Combining these two ideas we synthesize novel red coloured colloidal silver nanoparticle without destroying the Vigna mungo seeds. Rather than going with the general idea that green method will produce less toxic nanoparticle, we evaluate the toxicity in germinating seeds and RAW cell line along with antimicrobial and chemotaxis test. The result suggests that silver nanoparticles are antibacterial positive without chemotactic property. The cytotoxicity against RAW 246.7 murine macrophage cell linewas found high. Phytotoxicity was also found potent in case of Vigna mungo (dicot) and Oryza sativa (monocot) seed with respect to inhibition of germination.

Keywords: Silver Nanoparticle; Vigna Mungo; Toxicity; Antibacterial; Chemotaxis.