The advancement in technologies and machineries which have made the living more easy, on the contrary have caused people to be less active and follow a sedentary lifestyle. Call centers have emerged as the most sought after workplace for Indian women in recent times. Due to intensive work pressure, requiring high levels of concentration, the performance of employees deteriorates, also performing one type of activity throughout the day. Long hours of work, permanent night shifts, incredibly high work targets, and health problems are major issues. 75% of women working in a call centers have direct effect on their health as working night shifts was upsetting their biological clock. In this study 300 call centre female employees were selected randomly for the purpose through medical assistance in the form of physical health check up camp arranged in the vicinity of call centre. The information related to their health was collected through a questionnaire and physical screening. Descriptive statistics such as mean, frequencies and % were used to describe the association of the variables. The study revealed that majority of females possess normal blood pressure (77.3%), the problem of vision was higher in number (50%) as compare to hearing and earache problem i.e.(32.3% & 17.7%). It was found that the prevalence of bone problems was also higher i.e. (82%) in females which included shoulder joint pain, wrist joint, and neck-back pain. The acidity rate was slightly higher i.e. 44% whereas constipation problem was not much common in females (29%). Majority of the respondents reported of occupational stress and lack of sleep respectively, (63% and 53.3%). Social health was also affected in fifty per cent of the females (51.3%) and irregularity of menstrual cycles also reported in maximum number of females (79.3%).