Canine parvovirus is one of the most devastating viral disease of dogs causing high morbidity and mortality. Parvovirus proliferates mainly in the gastrointestinal tract of adults and myocardium in case of ups. Two clinical cases were presented to the teaching veterinary clinical complex with a history of dogs of age group 3 months, having a temperature of 1010F, diarrhea with foul odors and the dogs were not yet vaccinated. The clinical cases suspected of parvoviral infection were preliminarily screened by haemagglutination test and later confirmation was done by employing Polymerase Chain Reaction for the suspected fecal samples.